We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

Overview
Images
Techniques
Article Snippets
home > search results > product details
star_border
     Loading Product Details ...      Welcome to Your Next Discovery   
93 / 100
Bioz Stars
Caldicellulosiruptor bescii
DSMZ  
Product Name
Saccharomyces cerevisiae Meyen ex E C Hansen
Catalog Number
4017153
Biosafety Level
1
Genotype
MATalpha his3delta1 leu2delta0 lys2delta0 ura3delta0 yar002c-a::KanMX4
Category
Fungi
Mating Type
alpha
More Catalog Fields ...
Open an account
already a user? Sign in
are you a supplier? Contact us
Product Images (3) - All
All
Amplification
Construct
Expressing
Functional Assay
Marker
Methylation
Plasmid Preparation
Sequencing
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 
star_border
(A) A diagram of the pyrBCF locus in the C. <t>bescii</t> chromosome. The line above the diagram depicts the extent of the spontaneous deletion in the Δ pyrBCF strain. pDCW70 contains the <t>wild</t> <t>type</t> pyrBCF alleles with an engineered KpnI site used to select marker replacement of the deletion depicted with primers used to confirm the structure of the chromosome in the transformant. B) Δ pyrBCF electrocompetent cells after electro-pulse with no DNA added plated onto defined medium + uracil (top plate), Δ pyrBCF electrocompetent cells after electro pulse with unmethylated pDCW70 DNA plated onto defined medium w/o uracil (middle plate), Δ pyrBCF electrocompetent cells after electro-pulse with M.CbeI methylated pDCW70 DNA plated onto defined medium w/o uracil (bottom plate). (C) PCR products amplified using primers DC163 and DC188 (upper gel) digested with KpnI (lower gel). M: 1kb DNA Ladder (NEB). lane 1: amplified from wild type cells (3.2 kb); lane 2: amplified from Δ pyrBCF (1.63 kb); lane 3: amplified from transformants (1.9 and 1.3 kb cleavage products).
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 
star_border
Strains/plasmids used and constructed in this study.
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 
star_border
Predicted functional domains of M.CbeI and sequence alignments of conserved motifs of three M.CbeI homologues from members of <t>Caldicellulosiruptor</t> species as well as DmtB from Anabaena variabilis , which also contains an M.CbeI homologue showing unique features of this methyltransferase.
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 PLoS ONE, 2022 Dec 21
"(A) A diagram of the pyrBCF locus in the C. <t>bescii</t> chromosome. The line above the diagram depicts the extent of the spontaneous deletion in the Δ pyrBCF strain. pDCW70 contains the <t>wild</t> <t>type</t> pyrBCF alleles with an engineered KpnI site used to select marker replacement of the deletion depicted with primers used to confirm the structure of the chromosome in the transformant. B) Δ pyrBCF electrocompetent cells after electro-pulse with no DNA added plated onto defined medium + uracil (top plate), Δ pyrBCF electrocompetent cells after electro pulse with unmethylated pDCW70 DNA plated onto defined medium w/o uracil (middle plate), Δ pyrBCF electrocompetent cells after electro-pulse with M.CbeI methylated pDCW70 DNA plated onto defined medium w/o uracil (bottom plate). (C) PCR products amplified using primers DC163 and DC188 (upper gel) digested with KpnI (lower gel). M: 1kb DNA Ladder (NEB). lane 1: amplified from wild type cells (3.2 kb); lane 2: amplified from Δ pyrBCF (1.63 kb); lane 3: amplified from transformants (1.9 and 1.3 kb cleavage products). "
Techniques (20)
Plasmid PreparationPlasmid Preparation15.04%
SequencingSequencing14.20%
IsolationIsolation14.20%
ModificationModification13.93%
Protocol Conditions
Article Snippets for All Techniques